Trupti Patil Cell biologist, curious by nature who enjoys developing new ideas and building new skillsets. Skills: CELL BIOLOGY: Mammalian cell culture (cancer cell lines, EXPI293s), Cell based assays for GPCR functional characterization- cAMP modulation, Receptor internalization, Recycling, competition binding assay LNP/Delivery formulation characterization- Uptake and expression kinetics, Nuclear delivery assay, in-vitro immune toxicity (THP-1, Spenocytes)- Cytometric bead array, Luminex, Cell viability/proliferation assay, Flow cytometry, Stable Cell line development (Lentiviral) MOLECULAR BIOLOGY: Plasmid/nanoplasmid construct design, molecular cloning (Restriction enzyme, PCR, gibson cloning), DNA/RNA purifications/extractions PROTEIN: SDS PAGE, Western blot, Mammalian cell protein expression (EXPI293) and Purification (Affinity, size exclusion, FPLC)
Research Intern
Vocational, Biology
Masters, Engineering
